ABSTRACT

Isolation of MAP from breast milk of Crohn's disease patients

Naser SA¹, Schwartz D¹, Shafran I^2
1Department of Molecular Biology and Microbiology Center for Discovery of Drugs and Diagnostics, University of Central Florida, Orlando, Florida; ²Florida Hospital, Orlando, Florida

Received Nov. 10, 1999; accepted Nov. 30. 1999

This report is the first to describe the isolation of MAP from the milk of humans with CD. It also describes a practical protocol for short-term cultivation of MAP from clinical specimens. The finding adds more support to the MAP role in CD pathogenesis. Interestingly, the data further demonstrates the gross similarity between humans diagnosed with CD and Johne's disease mammals infected with MAP.

PARA's SUMMARY

VIABLE, POTENTIALLY INFECTIVE MAP HAVE BEEN ISOLATED FROM BREAST MILK OF WOMEN WITH CD. DEMOMSTRATING, BY ITS EXCRETION IN THEIR MILK, THE PRESENCE OF MAP IN CD PATIENTS.